By blue-white screening and sequence analysis, we obtained the positive clone . 2 . we constructed plant binary expression vector carrying fusions of the enhanced camv 35s promoter and dreb1c ( 35s : dreb1c ) in the sense orientation 我們從擬南芥基因組中克隆了dreb1c基因全長,并將其連接到中間載體pgem-t-easy上,進行了測序驗證,結(jié)果顯示所克隆的dreb1c序列完全正確。